The bioweapon is in the post / Did U.S. government lie about deadly virus? (11/11/2005)


1.The bioweapon is in the post - New Scientist
2.Did U.S. government lie about deadly virus? - World Science

They're so precautionary over bird flu, meanwhile...

"Terrorists could order genes that confer virulence to Ebola, say, and engineer them into another virus or bacterium" (item 1)

"I don't understand the logic of creating a threat so we can learn to defend against that threat, that would not have existed in the first place if we hadn't created it." (item 2)

1.The bioweapon is in the post
09 November 2005
NewScientist.com news service

YOU might think it would be difficult for a terrorist to obtain genes from the smallpox virus, or a similarly vicious pathogen. Well, it's not. Armed with a fake email address, a would-be bioterrorist could probably order the building blocks of a deadly biological weapon online, and receive them by post within weeks.

That's the sobering reality uncovered by a New Scientist investigation into the bioterror risks posed by the booming business of gene synthesis. Dozens of biotech firms now offer to synthesise complete genes from the chemical components of DNA (See "A dollar a base pair" [below]). Yet some are carrying out next to no checks on what they are being asked to make, or by whom. It raises the frightening prospect of terrorists mail-ordering genes for key bioweapon agents such as smallpox, and using them to engineer new and deadly pathogens.

Customers typically submit sequences by email or via a form available on a company's website. The companies then construct the specified genes and mail them back a few weeks later, usually spliced into a bacterium such as Escherichia coli. New Scientist approached 16 such firms, identified by a Google search, to ask whether they screened orders for DNA sequences that might pose a bioterror threat. Of the 12 companies that replied, just five said they screen every sequence received. Four said they screen some sequences, and three admitted not screening sequences at all (see Table).

The risks posed by gene synthesis first hit the headlines in 2002, when a team from the State University of New York at Stony Brook made infectious polioviruses from synthetic DNA. And just last month, researchers with the US Centers for Disease Control and Prevention in Atlanta, Georgia, said that they had used similar means to recreate the virus that caused the 1918 flu (New Scientist, 8 October, p 16).

In theory, a terrorist group could try to emulate the latter feat, or create a virus such as Variola major, which causes smallpox. However, the Variola genome comprises some 190,000 base pairs of DNA, and while some companies will make sequences 20,000 or more base pairs long, an attempt to order all the genes necessary to launch a smallpox attack would probably arouse suspicion. "That would stand out from a technological point of view," suggests Drew Endy, a bioengineer at the Massachusetts Institute of Technology.

A more realistic risk is that terrorists could order genes that confer virulence to dangerous pathogens such as the Ebola virus, and engineer them into another virus or bacterium. They could also order genes for a hazardous bacterial toxin - although many of these are also available by isolating the microorganisms from the environment.

Virulence genes are typically no more than a few thousand base-pairs long. Their sequences are publicly available, so screening gene-synthesis orders for potential bioweapons shouldn't pose a huge challenge. Indeed, a company called Craic Computing, based in Seattle, has written open-source software called Blackwatch that does just that. It is used by one of the leading gene-synthesis companies, Blue Heron Biotechnology of Bothell, Washington.

Robert Jones, president of Craic Computing, says that Blackwatch "casts a wide net", comparing orders against sequences from organisms identified by the US government as "select agents" that raise bioterror concerns. But not all of these sequences are dangerous, and some customers may have the clearance to work with those that are. So even legitimate orders may be flagged up as suspicious, and that means companies must employ biologists to carefully examine any matches that crop up.

The need for expert human checks may be one factor deterring some companies from screening orders. Others like to reassure customers who may be worried about commercial confidentiality that their sequence data will remain secret. But whatever the reasons, some firms freely admit that they run no sequence screens. "That's not our business," says Bob Xue, a director of Genemed Synthesis in South San Francisco.

Even if they don't routinely perform sequence checks, some companies say that they do investigate their customers. But the scope of these checks varies widely. While some firms say they conduct thorough probes into customers' affiliations and scientific publications, others are less exhaustive. For instance, Jennifer Wang, general manager of Bio Basic, based in Markham, Canada, says that her company examines email addresses to see if orders come from a legitimate research organisation.

Such a check would have spotted one suspicious order, sent from a Hotmail address to BaseClear of Leiden, the Netherlands. This was for a modified sequence from a hepatitis-like virus. BaseClear itself rejected the order after the would-be customer failed to respond to requests for more information, says Gerben Zondag, the firm's scientific director.

But email addresses are notoriously easy to fake. And even orders from legitimate institutions may not be what they seem. Alfred Lasher, who manages Picoscript in Houston, Texas, says that he turned down one order placed by an individual at a US biotech firm, after Picoscript's enquiries revealed the gene was being ordered on behalf of a friend in another country.

Experts are concerned that the checks currently employed by some companies aren't sufficient to exclude orders placed by terrorists. "We're taking this very seriously," says Endy. Together with the J. Craig Venter Institute in Rockville, Maryland, and the Center for Strategic and International Studies in Washington DC, Endy's research group at MIT has launched a study into the risks and benefits of synthetic genomics, and aims to produce a set of policy recommendations by late 2006. The US National Science Advisory Board for Biosecurity, set up last year to advise the US government on which advances in biology could be exploited by terrorists, is also considering the issue.

Some gene synthesis companies say they would welcome more detailed rules. John Mulligan, president of Blue Heron, says it would be helpful to have a list of "select sequences" that are off-limits for gene synthesis without explicit government permission, rather than having to make difficult judgments based on the list of select agents. "Tell us what we can't make," he implores.

But with gene synthesis firms springing up all over the world, and the underlying technology becoming cheaper and more widely available, it is unclear whether regulations enacted in any one country will be enough. "It's going to be virtually impossible to control," predicts David Magnus, director of the Stanford Center for Biomedical Ethics in Palo Alto, California.

Endy argues that what's needed is better self-regulation: if researchers only do business with companies that are diligent in sequence screening and other security checks, then terrorists would soon find themselves unable to place orders for dangerous genes. Otherwise, he fears a crackdown that could close valuable avenues of research. For instance, gene synthesis can be used to make DNA vaccines, which may eventually provide a means of responding rapidly to emerging diseases - or bioterrorist attacks.

"As soon as people start dying from a bioengineered organism, there will be a huge security response and research will be clamped down," warns Endy.

From issue 2525 of New Scientist magazine, 09 November 2005, page 8

A dollar a base pair

Biochemists have long known how to build DNA from its component "bases" - the chemical letters of the genetic code. By adding the bases in a prescribed order and carefully performing a series of chemical reactions, they can create precisely tailored stretches of DNA.

The process became significantly less laborious with the debut of the automated DNA synthesiser in the 1980s. But a full gene - a DNA sequence up to several thousand base pairs long - involves a formidable jigsaw puzzle.

Commercial gene synthesis has only really taken off in the past few years with advances in automating this assembly process. And as the main players jostle for position, the costs of gene synthesis are plummeting. Prices have dropped about tenfold in five years, and some firms now supply genes for less than $1.50 per base pair.

Reconstruction of 1918 flu virus prompts warnings

Virus synthesised in a fortnight

How the US crackdown on bioterror is backfiring

2.Did U.S. government lie about deadly virus?
World Science, Nov 9 2005

U.S. officials seem to have quietly reversed an assurance they gave publicly last month - that a deadly virus, which scientists recently recreated, would not leave a secure government facility.

Now, authorities acknowledge they perhaps will mail copies of the germ, which killed an estimated 50 million people in 1918, to qualified laboratories that apply for it.

The apparent flip-flop suggests the initial assurance might have been a lie, or deception, meant to calm a nervous public about the risky project, says the head of an anti-biological weapons organization.

But U.S. officials say they didn’t mislead anyone.

Scientists and government officials announced last month that they had designed a virus identical in most key respects to the infamous 1918 "Spanish Flu" virus.

The project’s stated purpose was to let scientists study the virus and thereby design vaccines against related pathogens, including a bird flu that is alarming governments worldwide.

But some experts expressed doubts from the start about the venture’s safety. They said the virus could accidentally escape or land in terrorist hands.

In response to such concerns, officials with the Centers for Disease Control and Prevention (CDC) in Atlanta, Ga., a U.S. agency, said the virus would be held securely at the agency's headquarters, and wouldn’t be sent elsewhere for research, according to newsreports.

The San Francisco Chronicle reported on Oct. 6 that Jennifer Morcone, a spokeswoman for the agency, had given such an assurance. If researchers from outside the agency want to work with the virus, the paper quoted her as saying, "We will consider hosting researchers at the CDC if they go through the same training and clearances required of our researchers."

The research journal Nature reported similar assurances by the officials. The Chicago Tribune cited CDC Director Julie Gerberding saying the agency had no plans to share the virus with other labs.

The apparent reversal, when it came, was quiet.

It appeared in the form of a cryptic notice - which the agency was legally required to publish - in the Oct. 20 Federal Register, the official publication of federal government notices.

It said the agency would add the virus to a "list of select agents and toxins" maintained by the Department of Health and Human Services.

Basically, this means the virus can be mailed out, agency spokesman Von Roebuck acknowledged, according to a news article in the Nov. 10 Nature.

"Labs that are registered to work with select agents—in particular, dangerous pathogens that are subject to specific handlingrules—will be able to request the virus," Nature reported, citing Roebuck. The parcels could travel via commercial carriers, the journal added.

A staff member who answered the phone at the CDC's media relations office on Tuesday told World Science that the agency hasn't announced the new policy publicly, as far as he knows.

The staffer, who identified himself as Chris Cox, referred further questions to Roebuck. Roebuck said in an emailed statement to World Science that the agency didn’t mislead anyone, because officials said only that they were not planning on sending out the virus.

He didn't deny it would ever happen, though. "Requests to obtain the virus for investigations at non-CDC laboratories that advance the science and understanding of influenza pandemics will be considered on a case-by-case basis ," he wrote, adding that such mailings will follow strict safety procedures.

The policy dismayed the project's critics.

Edward Hammond, director of the U.S. office of the Sunshine Project, a non-profit group that works against chemical and biological weaponsusage, said he wasn't sure whether the agency's original statement was a lie.

"Did they lie, as in did they know that they were going to flip this policy within a week? I don’t know - it's difficult to tell, but they certainly in my judgement deceived," he said.

On the other hand, he said, any expert on the subject would have known that the policy as originally stated was "a fiction to begin with." That's because even without the mailing, anyone with the right equipment could have reconstructed the virus using the information released as part of the project.

The no-mailing claim "was a red herring from the get-go," he said. "It was intended to reassure, when they knew that the assurance that most people would draw from it was based on a misunderstanding."

But the policy change raises the dangers still further, said Jens Kuhn, a research scholar at the acknowledged Harvard Medical School.

"There's a big risk associated with it," he said. He added that officials didn’t announce the mailing policy to begin with "probably because they would have gotten the same kind of heat they're getting now."

On the other hand, it might have been a good idea not to announce it, as this could further encourage bioterrorists, said Kuhn, who, like Hammond, opposed the project from the start.

"I don't understand the logic," he said, "of creating a threat so we can learn to defend against that threat, that would not have existed in the first place if we hadn’t created it."

Source: world-science.net

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